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在pH 4.0的B-R缓冲溶液中,速灭威、残杀威和呋喃丹能够产生内源荧光,但光谱严重重叠。当波长差Δλ=30 nm时,加入一定量的β-环糊精,3种农药的荧光强度均有不同程度的加强,且荧光强度与农药的浓度呈良好的线性关系,速灭威、残杀威和呋喃丹单组分的测量线性范围分别为0.2~2.0、0.02~0.38和0.04~0.56μg/mL;检出限分别为0.083、0.015和0.020μg/mL。应用多种化学计量学方法,如经典最小二乘(CLS)、主成分回归(PCR)和偏最小二乘(PLS)对同步荧光光谱严重重叠的3种农药混合体系合成样进行光谱解析并比较其分析能力。结果表明,PLS预报各组分的结果较好,采用该模型分析了一些实际食品样品,获得满意的结果。
Veloban, propoxur, and carbofuran can generate endogenous fluorescence in a B-R buffer solution at pH 4.0 but the spectra overlap seriously. When the wavelength difference Δλ = 30 nm, a certain amount of β-cyclodextrin was added, the fluorescence intensities of the three pesticides were enhanced to some extent, and the fluorescence intensity showed a good linear relationship with the concentration of pesticides. The linear range of single component of Wei and Carbofuran was 0.2 ~ 2.0, 0.02 ~ 0.38 and 0.04 ~ 0.56 μg / mL, respectively. The detection limits were 0.083, 0.015 and 0.020 μg / mL, respectively. Spectral analysis of three pesticide mixture mixtures with severe overlap of synchronous fluorescence spectra by classical chemometrics methods such as classical least squares (CLS), principal component analysis (PCR) and partial least squares (PLS) was performed and compared Its analytical ability. The results showed that the results of PLS prediction of each component were good. Some real food samples were analyzed by this model and satisfactory results were obtained.