【摘 要】
：
脂肪酶是一类重要的水解酶,广泛应用于食品、医药、化工等领域.脂肪酶通过固定化可以提高酶的使用效率.文章采用戊二醛共价连接法将来源于地衣芽孢杆菌(Bacillus licheni orm
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脂肪酶是一类重要的水解酶,广泛应用于食品、医药、化工等领域.脂肪酶通过固定化可以提高酶的使用效率.文章采用戊二醛共价连接法将来源于地衣芽孢杆菌(Bacillus licheni ormis)的胞外脂肪酶固定于氨基型载体ZH-HA之上,将1 9载体置入10mL酶液中反应3h后固定化酶活力达到最高为60U/g湿载体.经固定化后该酶的温度稳定性得到显著提升,当反应温度为45℃时最高酶活力达到220U/g湿载体.同时,固定化使酶在碱性条件下的稳定性得到提高,最适反应pH值为10.通过自制柱式反应器考察该酶工作稳定性,经过15批连续水解反应,固定化脂肪酶仍保持90％的活力,展现出良好的稳定性.
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