采用组织培养和单层细胞培养两种方法对ＳＤ大鼠的胰腺进行培养。在培养过程中，培养液上清中免疫活性胰岛素（ＩＲＩ）含量均处于较高水平，培养１５天后不仅其胞内胰岛素含量分别为＞８００ｎｇ／４０ｍｇ组织和＞８００ｎｇ／１０７个细胞，而且所得的胰岛细胞对葡萄糖刺激具有灵敏的应答能力。组织培养１３天、单层细胞培养７天后，培养液中即难以检出淀粉酶含量，可见这两种方法均能获得生长良好的胰岛细胞，且其胰岛素生物合成能力和应答能力均处于旺盛状态。 Tissue culture and monolayer culture were used to culture the SD rat pancreas. During the culture, the content of immunoreactive insulin (IRI) in the culture supernatant was at a high level. After 15 days of culture, not only the intracellular insulin contents were> 800 ng / 40 mg tissue and> 800 ng / 107 cells, respectively, Islet cells have a sensitive response to glucose stimulation. Tissue culture for 13 days, single cell culture for 7 days, the culture medium that is difficult to detect amylase content, showing that both methods can get well-islet cells, and insulin biosynthesis ability and responsiveness are in a state of exuberance .