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目的探讨高脂肪膳食诱导的肥胖大鼠学习记忆能力变化。方法健康雄性SD大鼠50只,随机选取10只,通过皮下注射D-半乳糖,建立大鼠学习记忆损伤模型,喂基础饲料(阳性对照组),其余动物高脂肪饲料连续喂养2周后,根据体重筛选出基础组大鼠10只,喂基础饲料,其余大鼠继续喂高脂肪饲料,10周后,再根据体重筛选出肥胖大鼠10只,采用Morris水迷宫方法检测大鼠学习记忆能力,处死动物后,测量体脂肪含量,收集血清,检测血脂指标。结果肥胖大鼠体重、肾周脂肪、睾周脂肪、网膜脂肪、体脂肪含量均明显高于基础组大鼠,血脂水平无明显变化;阳性对照组大鼠第3象限平均逃避潜伏期(18.54±2.73)s和总路程(298.60±48.18)cm明显高于基础组[分别为(8.27±1.82)s、(124.85±29.17)cm](P<0.05);与基础组比较,肥胖组大鼠第3象限平均逃避潜伏期和总路程[分别为(9.72±2.19)s、(166.31±37.12)cm]具有升高趋势。各组大鼠穿越平台次数差异无统计学意义。结论高脂肪膳食诱导的肥胖大鼠可能存在学习能力损伤。
Objective To investigate the changes of learning and memory abilities in obese rats induced by high fat diet. Methods Fifty healthy male Sprague-Dawley rats were randomly selected. Ten rats were randomly divided into three groups. The model of learning and memory impairment was established by subcutaneous injection of D-galactose. The rats were fed with basal diet (positive control group) Ten rats in the basic group were screened out according to body weight. The rats in the basal diet were fed with the basal diet. The remaining rats were fed with high-fat diet. Ten weeks later, ten obese rats were screened out according to body weight. Morris water maze test was used to detect learning and memory ability , After the animals were sacrificed, body fat content was measured, serum was collected, and blood lipids were measured. Results The body weight, perirenal fat, perihepatic fat, omental fat and body fat in obese rats were significantly higher than those in the basal rats, and there was no significant change in the blood lipid levels. The average escape latency in the third quadrant of the rats in the positive control group was 18.54 ± 2.73) s and total distance (298.60 ± 48.18) cm were significantly higher than those in the basal group [(8.27 ± 1.82) s, (124.85 ± 29.17) cm], respectively The average escape latency and total distance of the three quadrants [(9.72 ± 2.19) s, (166.31 ± 37.12) cm] showed an increasing tendency. There was no significant difference in the number of rats passing through the platform between each group. Conclusion High fat diet-induced obese rats may have learning ability impairment.