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分枝数是影响油菜产量的重要株型性状之一。为了有助于油菜分枝数的分子标记辅助育种,以甘蓝型油菜品系888-5(多分枝)和M083(少分枝)杂交形成的重组自交系(RIL)群体为材料,通过利用第一张油菜60KSNP芯片对群体进行高通量SNP分型,并结合单环境和多环境2种QTL检测方法对RIL群体在4个环境(武汉-2012、武汉-2013、扬州-2012和扬州-2013)下分枝数进行QTL定位。结果表明:共检测出18个分枝数QTL,分布于A2、A6、A7、C1和C4连锁群。其中11个QTL在2个以上环境下可重复检测到;有2个QTL与环境之间存在互作效应。主效QTL 2个(qBN2-3和qBNE2-1),分别在3个、4个环境下重复检测到,可解释的表型变异为13.12%~20.60%,2.80%~30.10%。qBNE2-1与环境存在互作效应。另外,通过利用SNP标记侧翼序列和油菜基因组比对作图,从3个QTL(qBN2-1、qBN7-6和q BN7-8,三者可解释的表型变异分别为19.40%~17.30%、5.70%~12.21%和7.88%~10.32%)的基因组区段内(分别为279kb、165kb和562kb)共筛选出4个与分枝数有关的候选基因,它们的拟南芥同源基因(分别为CUC2、PIN3、F23N20.8和PIN4)均参与拟南芥分枝数的分化或形态建成。
The number of branches is one of the important plant-type traits that affect rapeseed production. In order to facilitate molecular marker-assisted breeding of rapeseed branches, a recombinant inbred line (RIL) population formed by crossbreeding of 888-5 (many branches) and M083 (few branches) of Brassica napus materials was used as materials. A rapeseed 60KS NP chip was used for high-throughput SNP genotyping. In combination with single and multi-environment QTL detection methods, the RIL population was analyzed in four environments (Wuhan -2012, Wuhan -2013, Yangzhou-2012 and Yangzhou-2013 ) Under the number of branches QTL mapping. The results showed that 18 branch number QTLs were detected and distributed in linkage groups A2, A6, A7, C1 and C4. Eleven QTLs were detected repeatedly in two or more environments; two QTLs interacted with the environment. Two major QTLs (qBN2-3 and qBNE2-1) were detected repeatedly in three and four environments, respectively. The explained phenotypic variation ranged from 13.12% to 20.60% and 2.80% to 30.10%, respectively. qBNE2-1 interacts with the environment. In addition, the three QTLs (qBN2-1, qBN7-6 and q BN7-8) explained the phenotypic variation of 19.40% ~ 17.30%, respectively, by using SNP marker flanking sequences and rape genome mapping. 5.70% ~ 12.21% and 7.88% ~ 10.32%) of the genome segments (279kb, 165kb and 562kb, respectively) were screened out four candidate genes related to the number of branches, their Arabidopsis homologous genes CUC2, PIN3, F23N20.8 and PIN4) were all involved in the differentiation or morphogenesis of Arabidopsis thaliana.