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核苷酸类化合物与二价金属离子生成配合物的反应,在生物体内起着重要作用。许多科学工作者采用不同的手段,对这类反应进行了研究。但是由于实验方法和实验条件上的差异,所得结果亦不尽相同。Mg~(2+)和Ca~(2+)作为ATP酶的激活剂,参与ATP酶催化水解ATP的反应。一般认为该反应的机理是ATP先与Mg~(2+)、Ca~(2+)生成配合物ATPMg~(2-)与ATPCa~(2-),然后被催化水解。本文采用微量量热法,研究了这一中间过程。对于ATP-Mg~(2+)体系测定的结果,与在相似实验条件下用微量量热法得到的文献值相吻合,验证了数据的可靠性。对于ATP-Ca~(2+)体系,
The reaction of nucleotide compounds with divalent metal ions to form a complex plays an important role in living organisms. Many scientists use different means to study this type of response. However, due to differences in experimental methods and experimental conditions, the results obtained are not the same. As an activator of ATPase, Mg 2+ and Ca 2+ are involved in the ATP-catalyzed hydrolysis of ATP. It is generally believed that the mechanism of this reaction is that ATP firstly forms ATPMg ~ (2-) and ATPCa ~ (2-) complexes with Mg ~ (2 +) and Ca ~ (2+) and then catalytically hydrolyzes. In this paper, using the calorimetry method to study this intermediate process. The results of the determination of ATP-Mg ~ (2+) system are consistent with the literature values obtained by microcalorimetry under similar experimental conditions, which verifies the reliability of the data. For ATP-Ca 2+ system,