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目的:建立琥乙红霉素静脉乳剂的含量测定方法。方法:采用Agilent Zorbax SB-C_(18)柱(250 mm×4,6 mm,5μm),流动相为乙腈-0.02 mol·L~(-1)醋酸铵缓冲盐溶液(65:35),检测波长215 nm,流速为1.0 ml·min~(-1),柱温25℃,进样量20μl。结果:琥乙红霉素在60～600μg·ml~(-1)的浓度范围内与峰面积呈良好的线性关系(r=0.999 1),平均回收率为97.92%,RSD为0.43%(n=9)。结论:该方法简便、快速、准确,可用于琥乙红霉素的含量测定及稳定性分析。
Objective: To establish a method for the determination of erythromycin ethylsuccinate emulsion. METHODS: Agilent Zorbax SB-C 18 column (250 mm × 4,6 mm, 5 μm) was used as the mobile phase. The mobile phase was acetonitrile-0.02 mol·L -1 ammonium acetate buffer solution (65:35) The wavelength was 215 nm and the flow rate was 1.0 ml · min ~ (-1). The column temperature was 25 ℃ and the injection volume was 20μl. Results: Erythromycin ethylsuccinate had a good linear relationship with the peak area (r = 0.999 1) in the concentration range of 60 ~ 600μg · ml ~ (-1), the average recovery was 97.92% and the RSD was 0.43% (n = 9). Conclusion: The method is simple, rapid and accurate and can be used for the determination and stability analysis of erythromycin ethylsuccinate.