经１×１０－６ｍｏｌ／Ｌ视黄酸诱导的Ｐ１９细胞体外可向神经方向分化，接种于多聚赖氨酸（ｐｏｌｙＤｌｙｓｉｎｅ）和纤连蛋白（ｆｉｂｒｏｎｅｃｔｉｎ）包被的玻片后，细胞逐渐聚集成团，此时细胞的贴壁性较差，进行原位分子杂交时容易脱落。我们尝试在细胞表面覆盖一层明胶，减少了细胞的脱落，又比较了蛋白酶Ｋ和胃蛋白酶对细胞蛋白质的消化作用，确定胃蛋白酶可较温和地消化细胞蛋白质，使探针有效地透入结合，杂交后细胞亦能较完整地保留于玻片上。 P19 cells induced by 1 × 10-6mol / L retinoic acid could differentiate into neurons in vitro. After inoculation with polyDlysine and fibronectin coated slides, Gradually gathered into a group of cells, cell adhesion at this time poor, in situ hybridization is easy to fall off. We tried to cover the cell surface with a layer of gelatin, reducing cell shedding, compared with the digestive effect of proteinase K and pepsin on cellular proteins, confirming that pepsin can digest cell proteins more gently, allowing probe penetration into the binding After hybridization cells can also be more intact on the glass slide.