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目的以来源广泛、原植物药用成分量可测定的刺五加叶柄为外植体,研究刺五加体细胞胚胎发生情况,为实现药用成分高产刺五加的快速繁殖奠定基础。方法以3年生植株萌发15 d以内的刺五加复叶叶柄为外植体,考察2,4-D和BA对刺五加体细胞胚胎发生的影响。结果28 d培养后,2,4-D 1.5 mg/L+BA 1.0 mg/L处理的叶柄外植体中71.4%直接产生或经由愈伤组织间接产生了8.5个体胚。两种产生方式均可在诱导培养基中进行,但间接发生比例较少。转入相同或降低2,4-D质量浓度的培养基后,体胚渐次发育成熟。同时新的体胚也在逐渐产生,经由间接途径产生的体胚所占比例也随之上升。结论刺五加可以通过萌发15 d以内的复叶叶柄外植体实现体细胞胚胎发生,体胚诱导率取决于2,4-D和BA的质量浓度。
Objective To investigate the occurrence of somatic embryogenesis in Acanthopanax senticosus using a wide range of sources and the petiole of Acanthopanax senticosus, which can be used for determining the amount of original medicinal components, to lay the foundation for the rapid propagation of Acanthopanax Senticosus with medicinal ingredients. Methods The petioles of Acanthopanax senticosus were used as explants within 3 days of 3 year old plants. The effects of 2,4-D and BA on somatic embryogenesis of Acanthopanax senticosus were studied. Results After 28 days of culture, 71.4% of petiole explants treated with 2,4-D 1.5 mg/L+BA 1.0 mg/L directly generated or indirectly produced 8.5 individual embryos via callus. Both modes of production can be performed in the induction medium, but the indirect occurrence rate is less. After transferring into the medium with the same or lower 2,4-D mass concentration, somatic embryos gradually matured. At the same time, new somatic embryos are gradually emerging, and the proportion of somatic embryos produced via indirect pathways also increases. Conclusion Acanthopanax senticosus can achieve somatic embryogenesis through the petiole explants within 15 days after germination, and the somatic embryo induction rate depends on the mass concentration of 2,4-D and BA.