论文部分内容阅读
研究的目的是设计一种培养方案并使其达到最佳状态,以便用于人类囊胚的培养发育。 研究包括53例获卵>8个,受精卵>5个的患者,其年龄<40岁,曾进行过0～10次体外受精(IVF)周期。促性腺激素释放激素激动剂(GnRHa)的应用包括长方案及短方案。Metrodin HP(高纯促卵泡成熟激素FSH)的应用为150～600 IU/d,可达14 d。当超过3个卵泡直径≥16 mm时注射人绒毛膜促性腺激素(hCG)5 000 IU,36 h后取卵。取卵日起每日阴道置黄体酮400 mg共16 d或仅14 d,但结合hCG 1 000 IU于取卵日后4、7、10及13 d给与或仅用hCG 2 000IU于取卵后3、6、9 d给与以支持黄体。用
The purpose of the study was to design a culture protocol and to optimize it for use in the development of human blastocysts. The study included 53 patients with> 8 eggs and> 5 fertilized eggs, aged <40 years, having undergone 0 to 10 IVF cycles. Applications of gonadotropin-releasing hormone agonists (GnRHa) include long-term and short-term regimens. Metrodin HP (high purity follicle stimulating hormone FSH) application of 150 ~ 600 IU / d, up to 14 d. Human chorionic gonadotropin (hCG) was injected at 5,000 IU when more than 3 follicles were ≥ 16 mm in diameter, and eggs were taken after 36 h. Daily vaginal administration of 400 mg of progesterone daily for 16 d or 14 d after ovulation day but with hCG 1000 IU at day 4, 7, 10 and 13 after ovulation or only with hCG 2000 IU after ovulation 3, 6, 9 d to support luteal phase. use