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目的探讨哺乳动物雷帕霉素靶蛋白(m TOR)、缺氧诱导因子-1α(HIF-1α)及血管内皮生长因子(VEGF)在哮喘患儿外周血清中的表达及意义。方法随机选取2015年1月-2016年4月在该院儿科门诊就诊或住院治疗的哮喘患儿64例,其中急性发作期哮喘患儿34例(发作组),缓解期哮喘患儿30例(缓解组);另选取同期在该院治疗的支气管肺炎患儿31例(肺炎组),在儿童保健科健康体检儿童31例(对照组)。采用酶联免疫吸附(ELISA)法测定各组儿童血清中m TOR、HIF-1α及VEGF的表达水平。结果发作组m TOR、HIF-1α及VEGF水平均显著高于缓解组、肺炎组及对照组,差异均有统计学意义(均P<0.01);缓解组m TOR、HIF-1α、VEGF水平均显著高于肺炎组及对照组,差异均有统计学意义(P<0.01);肺炎组与对照组间差异无统计学意义(P>0.05);发作组外周血中m TOR与HIF-1α水平呈正相关(r=0.919,P<0.01),m TOR与VEGF水平呈正相关(r=0.906,P<0.01),HIF-1α与VEGF水平呈正相关(r=0.925,P<0.01)。结论外周血m TOR、HIF-1α及VEGF表达水平升高与哮喘的发病相关,m TOR可能通过调节HIF-1α、VEGF的表达参与哮喘的发病过程。
Objective To investigate the expression and significance of mTOR, hypoxia inducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF) in peripheral blood of asthmatic children. Methods Sixty-four children with asthma were randomly selected from January 2015 to April 2016 in pediatric outpatient department or inpatient hospital, of which 34 were acute asthmatic children (seizure group), 30 were asthmatic children Remission group). Another 31 children with bronchopneumonia (pneumonia group) were treated in this hospital in the same period, and 31 healthy children (control group) in child health department. Serum levels of m TOR, HIF-1α and VEGF were measured by enzyme-linked immunosorbent assay (ELISA). Results The levels of mTOR, HIF-1α and VEGF were significantly higher in attack group than those in remission group, pneumonia group and control group (all P <0.01). The levels of mTOR, HIF-1α and VEGF in remission group (P <0.01). There was no significant difference between the pneumonia group and the control group (P> 0.05). The levels of m TOR and HIF-1α in the peripheral blood in the attack group were significantly higher than those in the control group (R = 0.919, P <0.01). There was a positive correlation between mTOR and VEGF levels (r = 0.906, P <0.01). There was a positive correlation between HIF-1α and VEGF (r = 0.925, P <0.01). Conclusion The increased expression of mTOR, HIF-1α and VEGF in peripheral blood is correlated with the pathogenesis of asthma. M TOR may be involved in the pathogenesis of asthma by regulating the expression of HIF-1α and VEGF.