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目的:探讨卵子玻璃化冷冻在睾丸取精失败周期中的临床应用价值。方法:回顾分析体外受精周期取卵日因男方无精子症行睾丸取精失败且无供精接受卵子冷冻的8例不孕症妇女。所有卵子经玻璃化冷冻保存2个月后解冻,存活的成熟卵子采用与其丈夫血型一致的精子库冷冻精子行卵细胞胞质内单精子注射(ICSI)受精,记录卵子的存活、受精、卵裂、优质胚胎、临床妊娠及分娩情况。结果:冷冻-解冻60枚卵子中存活47枚,存活率为78.3%(47/60);其中41枚成熟卵子行ICSI受精,正常受精率为80.5%(33/41),卵裂率为81.8%(27/33),优质胚胎率为59.3%(16/27);15枚胚胎移植给8例患者,移植周期率为100%(8/8),平均移植胚胎数为1.9±0.8。B超显示有5个孕囊形成,胚胎种植率为33.3%(5/15);5例患者获得了临床妊娠,临床妊娠率为62.5%(5/8),均为宫内单活胎。4例已顺利分娩3男婴1女婴,体重为(3 787.5±513.7)g,染色体及发育均正常。结论:卵子玻璃化冷冻是睾丸取精失败患者的有效保障,与精子库冷冻供精受精可以获得理想临床结局。
Objective: To investigate the clinical value of vitrification of oocytes in the cycle of testicular debridement failure. Methods: Eight infertile women who underwent in vitro fertilization cycle oestrous ovulation and failed to fertilize testicular azoospermia were retrospectively analyzed. All the eggs were frozen in vitrification for 2 months and thawed. The surviving mature eggs were sperm bank frozen spermatozoa with her husband’s blood type. ICSI of egg cells was fertilized and the survival, fertilization, cleavage, Good quality embryo, clinical pregnancy and childbirth. Results: Forty-seven survived in frozen-thawed 60 eggs were 78.3% (47/60). Among them, 41 mature eggs were fertilized with ICSI, the normal fertilization rate was 80.5% (33/41) and the cleavage rate was 81.8 % (27/33). The rate of high-quality embryos was 59.3% (16/27). Fifteen embryos were transplanted to 8 patients. The rate of transplantation was 100% (8/8) and the average number of embryos transferred was 1.9 ± 0.8. 5 gestational sacs were formed by B-ultrasound, and the implantation rate of embryos was 33.3% (5/15). Clinical pregnancy was obtained in 5 patients. The clinical pregnancy rate was 62.5% (5/8), all of which were intrauterine single live fetus. In 4 cases, 3 baby boys and 1 baby girl were successfully delivered with a body weight of (3 787.5 ± 513.7) g and normal chromosomes and development. Conclusion: Egg vitrification is an effective guarantee for patients with failed testicular sperm injection, and cryopreserved with the sperm bank to obtain the ideal clinical outcome.