主要对牡丹籽甾醇的提取及其抗氧化活性进行了研究。通过响应面分析法确定牡丹籽甾醇提取的最佳工艺条件,即微波功率为359 W,提取时间为70 s,乙醇质量分数为90.2%,液料比值为31(m L/g),此条件下牡丹籽甾醇的提取率达到3.19 mg/g。牡丹籽甾醇具有较强清除OH·和O_2~-·的能力。随着牡丹籽甾醇浓度增加,其清除能力逐渐增强。当牡丹籽甾醇质量浓度大于0.9 mg/m L时,其对OH·的清除率大于柠檬酸和VC。当牡丹籽甾醇质量浓度达到1.8 mg/m L左右时,其对O_2~-·的清除率大于VC,且在较大程度上大于柠檬酸。因此,牡丹籽甾醇有较好的抗氧化活性。 The main extraction of peony sterol and its antioxidant activity were studied. The optimal conditions of peony sterol extraction were determined by response surface methodology, that is, the microwave power was 359 W, the extraction time was 70 s, the mass fraction of ethanol was 90.2% and the liquid-to-liquid ratio was 31 (m L / g) Under the peony sterol extraction rate of 3.19 mg / g. Peony sterol has a strong ability to clear OH · and O_2 ~ - ·. With the peony sterol concentration increased, its scavenging capacity gradually increased. When peony sterol concentration was more than 0.9 mg / m L, the scavenging rate of OH · was higher than that of citric acid and VC. When the mass concentration of peony sterol reached about 1.8 mg / m L, the clearance rate of O 2 - - was higher than that of VC, and to a greater extent than that of citric acid. Therefore, peony sterol has good antioxidant activity.