本文报道了用两性离子去垢剂EmpigenBB裂解Ⅰ型单纯疱疹病毒(HSV-1)感染的Vero或MRC-5细胞后获得的HSV-1制剂的免疫原性和特征。作者将5% Empigen PBS加入细胞致病作用达90%并经两次洗涤的HSV-1感染的细胞中,用超声破碎、100,000g离心和PBS透析,其上清即为粗制HSV-1抗原。将此粗制物再进行20%蔗糖密度梯度超速离心,收集顶端部分,于PBS中透析后,获得了部分纯 This article reports the immunogenicity and characterization of HSV-1 preparations obtained after cleavage of Vero or MRC-5 cells infected with Herpes Simplex Virus Type 1 (HSV-1) using the zwitterionic detergent EmpigenBB. The authors added 5% Empigen PBS to HSV-1-infected cells that had a cytopathic effect of 90% and were washed twice, sonicated, centrifuged at 100,000 g and PBS, and the supernatant was crude HSV-1 antigen . This crude material was then subjected to ultracentrifugation in a 20% sucrose density gradient, collecting the apical portion, dialyzing in PBS to obtain a partially pure