一、酶固定化的电聚合条件选择1不同介质对固定化酶活性的影响描次数30次,溶液中二茂铁甲酸浓度为0.2mmol·L~(-1)。三、介体酶传感器的性能由上述条件制备的葡萄糖氧化酶介体传感器的线性范围为3.2×10~(-8)-3.2×10~(-3)mol·L~(-1),响应时间约70秒,在pH6.0-9.0内对响应电流无影响,一个月内酶活性无变化,二个月后,酶活性下降一半,但仍可使用。溶解氧对测定无影响,测定电位为0.4V,10~(-5)mol·L~(-1)的抗坏血酸不干扰测定,测定的变动系数为0.25％。 First, the electrocatalytic immobilization of electropolymerization conditions 1 different media on the immobilized enzyme activity of the number of times described 30 times, the solution of ferrocene formic acid concentration of 0.2mmol·L -1. Third, the performance of the media enzyme sensor Glucose oxidase mediator prepared by the above conditions linear range of 3.2 × 10 -8 -8.2 × 10 -3 mol·L -1, the response Time of about 70 seconds, no effect on the response current within pH6.0-9.0, no change in enzyme activity within a month, two months later, the enzyme activity decreased by half, but can still be used. Dissolved oxygen had no effect on the determination. The determination potential was 0.4V. The determination of 10 -5 mol·L -1 ascorbic acid did not interfere with the determination. The coefficient of variation was 0.25%.