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用蓝桉(Eucalyptusglobulus)离体芽器官诱导培养,分化形成丛生芽,年繁殖系数3 ̄(12)。0.1~0.5mg/L的6-BA或0.5~0.8mg/L的KT诱导外植体(带节茎段)腋芽萌动的效果最佳,诱导率分别达80.3%和81.5%。1.5~20mg/L的6~BA或20~2.5mg/L的KT分别与0.5~1.0mg/L的NAA组合,对于促进腋芽分化形成丛生芽及继代培养中芽的增殖具有最佳效果。培养基中的无机盐浓度、蔗糖含量对蓝桉试管苗的生根具有显著影响;IBA促进蓝桉试管苗的生根。至目前为止,在1/2MS无机盐培养基+IBA1.2~1.4mg/L+S5g/L中诱导生根,生根率最高可达26.4%。
The explants of Eucalyptus globulus were induced to grow and differentiate into clusters of buds, with an annual reproductive coefficient of 3 ~ (12). The axillary buds germinated with 0.1-0.5 mg / L 6-BA or 0.5-0.8 mg / L KT induced explants with the stem segments had the best induction rate of 80.3% And 81.5%. 1.5 ~ 20mg / L of 6 ~ BA or 20 ~ 2.5mg / L of KT combined with 0.5 ~ 1.0mg / L of NAA, to promote the axillary bud differentiation into formation of buds and subculture buds Proliferation has the best effect. The concentration of inorganic salt in the medium and sucrose content had a significant effect on the rooting of Eucalyptus globulus seedlings. IBA promoted the rooting of Eucalyptus globulus seedlings. Up to now, the rooting was induced in 1 / 2MS inorganic salt medium + IBA1.2 ~ 1.4mg / L + S5g / L with the highest rooting rate of 26.4%.