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目的 :研究柴胡清肝汤对食物过敏小鼠肝脏细胞活素的影响。方法 :小鼠用卵蛋白致敏。卵蛋白 (Ova) 10 0 μg +铝剂 (作为增强佐剂 ) 0 .1mL腹腔注射 ,并用Ova 10 0 μg经口投与 ,每周 1次 ,共5次 ,第 3次致敏后动物分成 3组分别投与柴胡清肝汤、生理盐水 ,bid ,2wk。最后 1次致敏后 1wk ,用Ova(2 0 g·L- 1)经肠道攻击 ,3h后采取肝脏组织 ,免疫组织化学染色肝样本进行分析细胞活素IL4 ,IL6和TNFα。结果 :柴胡清肝汤 (10 0mg·kg- 1·d- 1和 30 0mg·kg- 1·d- 1)治疗组细胞活素IL4为 0 .4± 0 .2和 0 .7± 0 .2 ,IL6为 1.1± 0 .4和1.0± 0 .3,TNF α为 0 .8± 0 .3和 1.2± 0 .7均低于生理盐水组 [分别为 (8.4± 1.9) ,(11.6± 2 .4 ) ,(8.1± 1.9) ],差异有非常显著意义 ,P <0 .0 1。结论 :柴胡清肝汤可抑制食物过敏小鼠肝脏中的细胞活素
Objective: To study the effect of Chaihu Qinggan Decoction on hepatic cytokines in food-allergic mice. Methods : Mice were sensitized with ovalbumin. Ova (Ova) 100 μg + aluminum (as a reinforcing adjuvant) 0.1 mL intraperitoneal injection and Ova 100 μg orally once a week for 5 times, after the third sensitization, animals were divided into 3 The groups were given Chaihu Qinggan Decoction and saline, respectively, bid, 2wk. At the end of 1 sensitization after the last sensitization, Ova (2 0 g·L-1) was used for enteral challenge. After 3 hours, liver tissue was taken. Liver samples were immunohistochemically stained to analyze cytokines IL4, IL6 and TNFα. RESULTS: In the treatment group, the activity of IL4 in Chaihu Qinggan Decoction (10 0 mg·kg - 1·d - 1 and 300 mg·kg - 1·d - 1) was 0.4 ± 0.2 and 0.7 ± 0.2. IL6 was 1.1±0.4 and 1.0±0.3, TNF α was 0.8±0.3 and 1.2±0.7 were lower than that of saline group [(8.4±1.9), (11.6±2, respectively). .4) (8.1 ± 1.9)], the difference was significant, P <0. Conclusion : Chaihu Qinggan Decoction can inhibit the cytokines in the liver of food allergic mice