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目的:建立胃肠宁片中没食子酸、阿魏酸、槲皮素、山柰素、异鼠李素5个活性成分的HPLC含量测定方法。方法:采用Gemini C_(18)色谱柱(4.6 mm×250 mm,5μm),以甲醇(A)-0.4%的磷酸水溶液(B)为流动相进行梯度洗脱,流速0.8 m L·min~(-1),柱温30℃,检测波长为270 nm(没食子酸)和360 nm(阿魏酸、槲皮素、山柰素、异鼠李素)。结果:没食子酸、阿魏酸、槲皮素、山柰素、异鼠李素质量浓度分别在10.25~512.60、2.47~124.30、3.27~163.50、0.72~35.80、0.83~41.60μg·m L~(-1)范围内与峰面积呈良好的线性关系,加样回收率分别为100.0%、102.7%、97.9%、101.3%和98.8%;3批样品中没食子酸、阿魏酸、槲皮素、山柰素、异鼠李素含量范围分别为2 168.51~2 291.78、80.83~93.58、104.09~138.45、1 779~30.31、34.95~41.06μg·g~(-1)。结论:本试验所建立的方法可为胃肠宁片的质量控制提供科学的依据。
Objective: To establish an HPLC method for the determination of five active ingredients, ie gallic acid, ferulic acid, quercetin, behenicol and isorhamnetin in Weichangning tablet. Methods: The gradient elution was performed on a Gemini C 18 column (4.6 mm × 250 mm, 5 μm) using a mobile phase of methanol (A) -0.4% phosphoric acid (B) at a flow rate of 0.8 m L · min ~ -1), the column temperature was 30 ℃, the detection wavelength was 270 nm (gallic acid) and 360 nm (ferulic acid, quercetin, kaempferol, isorhamnetin). Results: The concentrations of gallic acid, ferulic acid, quercetin, kaempferol and isorhamnetin were 10.25 ~ 512.60, 2.47 ~ 124.30, 3.27 ~ 163.50, 0.72 ~ 35.80 and 0.83 ~ 41.60μg · m ~ -1), and the peak area was linear with the recoveries of 100.0%, 102.7%, 97.9%, 101.3% and 98.8%, respectively. The contents of gallic acid, ferulic acid, quercetin, The contents of sumatrin and isorhamnetin were 2 168.51 ~ 2 291.78, 80.83 ~ 93.58, 104.09 ~ 138.45, 1 779 ~ 30.31 and 34.95 ~ 41.06 μg · g -1, respectively. Conclusion: The method established in this study can provide a scientific basis for the quality control of Weichangning Tablet.