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本文应用体外细胞培养和斑点杂交技术,首次对重组人IL-6对人系膜细胞c-myc原癌基因mRNA的表达以及大黄素对IL-6的作用进行了观察。发现IL-6能够促进人系膜细胞c-myc原癌基因mRNA的增殖表达,该作用呈明显的剂量依赖性,而大黄素能够拮抗IL-6的上述作用。本项研究阐明了IL-6促进系膜细胞的可能机理,为进一步论证大黄素对系膜细胞功能的影响,大黄素与细胞因子间的相互作用以及大黄在防治肾小球硬化的作用奠定了基础。
In this paper, the expression of c-myc oncogene mRNA in human mesangial cells and the effect of emodin on IL-6 were observed for the first time by in vitro cell culture and dot blot hybridization. IL-6 was found to promote the proliferation of c-myc proto-oncogene mRNA in human mesangial cells in a dose-dependent manner. Emodin could antagonize the above effects of IL-6. This study clarified the possible mechanism of IL-6 promoting mesangial cells, in order to further demonstrate the emodin on mesangial cell function, the interaction between emodin and cytokines and rhubarb in the prevention and treatment of glomerular sclerosis laid the foundation basis.