目的:探讨体外受精(IVF)中异常的3原核(PN)胚胎的发育及可利用价值。方法:收集IVF治疗周期中废弃的3PN受精卵204个进行体外培养,观察其发育能力,并与同周期的1 138个2PN受精卵进行比较;采用胚胎植入前遗传学筛查(PGS)技术对由3PN发育成的19枚囊胚进行非整倍体分析。结果:3PN组和2PN组的卵裂率无统计学差异(P>0.05);但3PN组囊胚形成率显著低于2PN组[9.6%(19/97)vs 37.9%(204/342),P<0.01]。整倍体分析显示,10.5%(2/19)的3PN来源的囊胚为正常二倍体核型。结论:3PN受精卵有继续发育能力;囊胚培养和高通量测序可作为有效筛选异常PN受精卵中正常核型胚胎的一种方法。 Objective: To investigate the development and availability of abnormal 3 prokaryotic (PN) embryos in in vitro fertilization (IVF). Methods: Totally 204 3PN fertilized eggs in IVF treatment cycle were collected and cultured in vitro. The developmental ability of 3PN fertilized eggs was observed and compared with that of 1 138 2PN fertilized eggs in the same cycle. The preimplantation genetic screening (PGS) 19 blastocysts developed from 3PN were analyzed for aneuploidy. Results: The blastocyst rates of 3PN group and 2PN group were not significantly different (P> 0.05). However, the blastocyst formation rate of 3PN group was significantly lower than that of 2PN group [9.6% (19/97) vs 37.9% (204/342) P <0.01]. Euploid analysis showed that 10.5% (2/19) of 3PN-derived blastocysts were normal diploid karyotypes. CONCLUSION: The 3PN fertilized eggs have the ability of continued development. Blastocyst culture and high-throughput sequencing can be used as a method to effectively screen normal karyotype embryos in abnormal PN fertilized eggs.