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李喆氏等报道,用四氯化碳造成大鼠离体灌流肝损伤模型,观察了三七总皂甙(PNS)对该模型的作用,目的在于了解动物实验和离体器实验是否有一致性及对 PNS的保肝作用机理进行初步探讨。本实验结果表明:PNS 能增加大鼠离体灌流肝的灌流量,提示其保肝作用机理可能与其改善肝内微循环有关。在灌流液中加入 PNS 后,能明显抑制 GPT 的升高,灌流后肝组织的显微观察也提示,PNS 组肝损伤明显轻于 CCl_4损伤组。说明离体灌流肝模型能反映和观察到PNS 对化学性肝损伤的保护作用,与整体动物实验结果一致。该实验揭示在临床上使用三七治疗肝脏疾病提供了一定的实验依据。
Li et al. reported that a rat model of perfused liver injury was induced with carbon tetrachloride and the effects of Panax Notoginseng Saponins (PNS) on the model were observed. The purpose was to understand whether there was consistency between animal experiments and ex vivo devices. And preliminary discussion on the mechanism of liver protection of PNS. The results of this experiment show that PNS can increase the perfusion rate of isolated perfused liver in rats, suggesting that the mechanism of liver protection may be related to the improvement of intrahepatic microcirculation. The addition of PNS to the perfusate significantly inhibited the elevation of GPT. Microscopic observation of the hepatic tissue after perfusion also suggested that the liver injury in the PNS group was significantly lighter than that in the CCl_4 injury group. It shows that the isolated perfused liver model can reflect and observe the protective effect of PNS on chemical liver injury, which is consistent with the overall animal experimental results. The experiment revealed that the use of Panax notoginseng in the treatment of liver disease provides a certain experimental basis.