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目的探讨不同黑色素瘤细胞株对盐霉素的敏感性,及盐霉素对敏感细胞株细胞周期的影响。方法 8种不同黑色素瘤细胞株,采用不同浓度盐霉素(40、20、10、5、1.25、0.625、0.312 5μmol/L)作用3d,计算盐霉素对8种黑色素瘤细胞株的半数抑制浓度(half maximal inhibitory concentration,IC50),挑选对盐霉素敏感的黑色素瘤细胞株;将敏感黑色素瘤细胞株分为对照组、0.1μmol/L盐霉素组、0.3μmol/L盐霉素组和1.0μmol/L盐霉素组,分别给予0、0.1、0.3、1.0μmol/L盐霉素处理细胞,处理48h时采用流式细胞仪观察各组细胞周期变化情况,1.0μmol/L盐霉素组处理24、48h后观察细胞形态的变化情况。结果 SK-MEL-19细胞对盐霉素的IC50[(0.82±0.60)μmol/L]最低,低于其他7种细胞株,SK-MEL-19细胞对盐霉素最敏感;作用48h后,1.0μmol/L盐霉素组SK-MEL-19细胞G0G1期[(39.90±3.43)%]、G2M期[(4.03±1.51)%]、S期[(33.99±1.09)%]细胞比例明显低于对照组[(48.92±1.14)%、(6.39±0.77)%、(44.69±1.36)%]、0.1μmol/L盐霉素组[(44.65±1.13)%、(8.23±0.96)%、(47.12±1.18)%]和0.3μmol/L盐霉素组[(46.18±2.88)%、(8.20±2.17)%、(45.62±1.83)%](P<0.05),对照组及0.1、0.3μmol/L盐霉素组细胞周期比例两两比较差异均无统计学意义(P>0.05);1.0μmol/L盐霉素组作用24h后部分SK-MEL-19细胞出现空泡,作用48h后细胞出现明显死亡,且细胞周期有小凋亡峰(subG1峰)出现,有细胞凋亡存在。结论盐霉素对SK-MEL-19黑色素瘤细胞株有较强杀伤作用,此杀伤作用可能是通过诱导细胞凋亡实现的。
Objective To investigate the sensitivity of different melanoma cell lines to salinomycin and the effect of salinomycin on cell cycle of sensitive cell lines. Methods Eight different melanoma cell lines were treated with different concentrations of salinomycin (40, 20, 10, 5, 1.25, 0.625 and 0.312 5μmol / L) (IC50) were selected to select salinomycin-sensitive melanoma cell lines. The sensitive melanoma cell lines were divided into control group, 0.1μmol / L salinomycin group, 0.3μmol / L salinomycin group And 1.0μmol / L salinomycin group, respectively, treated with 0,0.1,0.3,1.0μmol / L salinomycin, treated with flow cytometry 48h when the cell cycle changes in each group, 1.0μmol / L Salinity Su-group 24 and 48h after treatment observed changes in cell morphology. Results SK-MEL-19 cells had the lowest IC50 of salinomycin (0.82 ± 0.60 μmol / L), lower than the other 7 cell lines. SK-MEL-19 cells were the most sensitive to salinomycin. After 48 hours, The proportion of G0G1 [(39.90 ± 3.43)%], G2M (4.03 ± 1.51)% and S [33.99 ± 1.09%] cells in SK-MEL-19 cells in 1.0 μmol / In the control group [(48.92 ± 1.14)%, (6.39 ± 0.77)%, (44.69 ± 1.36)%], 0.1μmol / L salinomycin group [(44.65 ± 1.13)%, (8.23 ± 0.96)%, (46.18 ± 2.88)%, (8.20 ± 2.17)%, (45.62 ± 1.83)%] (P <0.05) in the control group and 0.1, 0.3 μmol / L Salinomycin group showed no significant difference (P> 0.05). After treated with 1.0 μmol / L salinomycin for 24 hours, some SK-MEL-19 cells were vacuolized, and after 48 hours, There was obvious death, and there was a small apoptotic peak in the cell cycle (subG1 peak), with apoptosis. Conclusion Salinomycin has a strong killing effect on SK-MEL-19 melanoma cell lines, and its killing effect may be through the induction of apoptosis.