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笔者应用超滤浓缩和密度梯度区带离心技术,对Ｖｅｒｏ细胞制备的人用狂犬病疫苗进行了以蔗糖为介质的密度梯度区带离心纯化研究。1 材料和方法1.1 狂犬疫苗的制备 在10Ｌ转瓶常规培养的Ｖｅｒｏ细胞中接种ＣＴＮ-Ｖ1狂犬病固定毒,于37℃培养3ｄ后,换维持液;每过3～4ｄ收获病
The author used ultrafiltration concentration and density gradient zone centrifugation technology to study the rabies vaccine prepared by Vero cells in the density gradient zone with sucrose as the medium. MATERIALS AND METHODS A. Preparation of Rabies Vaccine CTN-V1 rabies virus was inoculated into Vero cells routinely cultured in 10L roller bottles and cultured for 3 days at 37 ℃. The maintenance liquid was harvested every 3 to 4 days