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从浙江各地、江苏南部和上海等地采集的西瓜枯萎病株和其他瓜类植株上,分离纯化尖孢镰孢霉(Fusarium oxysporum)菌种23个(每个菌种来自一个植株样本).接种试验表明:酉瓜植株上分离的19个菌种,有16个对长蜜、新红宝和圳宝等3个酉瓜品种表现为致病性,而其他菌种表现为非致病性.所有23个菌种均获得抗氯酸盐的硝酸盐营养突变株(nit mutant).根据来自同一菌种nit突变株的营养体亲和性与突变类型鉴定,选择3个西瓜致病菌的Nit M类型突变株(W 024～5,W 072～4和W 014～9)为营养体亲和性测试株.各菌种上获得的nit突变株分别与测试株作营养体亲和性配对反应,23个菌种可划分成8个营养体亲和群(VCG)和1个营养体自身非亲和类型.16个西瓜致病菌中,除一个为营养体自身非亲和类型外,其余均属同一亲和群(M1001),而与西瓜非致病菌不存在营养体亲和反应.由此显示:营养体亲和群与西瓜枯萎病菌存在相关性,而与地理分布无关.
Twenty-three Fusarium oxysporum strains were isolated and purified from each of the fusarium wilt plants and other melons collected from all over Zhejiang, southern Jiangsu and Shanghai (each from one plant sample). The results showed that 16 of the 19 bacterial species isolated from the squash plants exhibited pathogenicity against three squash cultivars such as Changmei, Xinhongbao and Zhenbao, while the other strains showed non-pathogenicity. All 23 strains were obtained nitrite salt resistant nitrate mutants.According to the same strain of nit mutants of vegetative compatibility and mutation type identification, select three watermelon pathogens Nit M type mutants (W 024 ~ 5, W 072 ~ 4 and W 014 ~ 9) were vegetative compatibility test strains.The mutants of nitrates obtained from each strain were respectively matched with the test strains for nutrient affinity matching reaction , 23 species can be divided into 8 vegetative affinity groups (VCG) and 1 vegetative non-affinitive type.Among 16 watermelon pathogenic bacteria, except for one of the vegetative body non-affinities, the rest All belong to the same affinity group (M1001), but not with watermelon non-pathogenic bacteria does not exist nutrient affinity reaction.Therefore, Melon wilt correlated germs, regardless of the geographic distribution.