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通过对 1 1例重型肝炎病人中扩增的 HBV DNA直接序列分析 ,研究重型肝炎病人中 HBV C基因的变异及其特点。每个病例的 HBV C基因均有数量不等的变异 ,产生 1～ 1 2个氨基酸替代。慢性重型肝炎病人的 HBV C基因变异明显多于亚急性重型肝炎病人。C基因的变异呈聚集性 ,相对集中于 T淋巴细胞和 B淋巴细胞的抗原识别位点。重型肝炎病人的 HBV C基因有较高的变异率 ,在慢性重型肝炎的病人明显高于亚急性重型肝炎病人 ,变异相对集中于 T淋巴细胞和 B淋巴细胞抗原识别位点 ,说明与疾病的慢性化和免疫清除相关
Through the direct HBV DNA sequence analysis of 11 cases of patients with severe hepatitis, we studied the variation of HBV C gene and its characteristics in patients with severe hepatitis. Each case of HBV C gene has a variable amount of variation, resulting in 1 to 12 amino acid substitutions. HBV C gene mutations in patients with chronic severe hepatitis were significantly more than those in patients with subacute severe hepatitis. The variation of C gene is aggregative, relatively concentrated in the antigen recognition sites of T lymphocytes and B lymphocytes. Patients with severe hepatitis C have a higher mutation rate of HBV C gene in patients with chronic severe hepatitis was significantly higher than those in patients with sub-acute severe hepatitis, the variation is relatively concentrated in T lymphocytes and B lymphocyte antigen recognition site, indicating that chronic disease Related to immune clearance