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以湘早籼8号为受体材料,以易于大量获得的鲤鱼精液DNA为分子供体,进行浸胚法DNA导入水稻实验,研究了DNA分子在浸胚过程中的降解情况。通过定时取样后琼脂糖凝胶电泳、紫外吸收光谱扫描分析,发现水稻胚在浸泡过程中能主动降解外源DNA分子,48h后大部分DNA分子被降解成小分子片段、单磷酸脱氧核苷酸、碱基甚或无机成分;紫外吸收光谱扫描发现220~240nm有明显的增色效应。鉴于浸胚法诱变优势的普遍性及基因导入的固有困难,推测外源DNA浸胚过程中降解后的成分对诱变起更大的作用。
Using Xianzaoxian 8 as acceptor material, DNAs of digestive tract were digested with embryo DNA into rice in order to study the degradation of DNA during embryogenesis. After timed sampling, agarose gel electrophoresis and UV-vis spectrum scanning analysis showed that rice embryos could actively degrade exogenous DNA molecules during soaking, and most of the DNA molecules were degraded into small fragments after 48h. Deoxyribonucleotide monophosphate , Base or even inorganic components; UV absorption spectroscopy found significant 220 ~ 240nm enhancement effect. In view of the universality of mutagenesis advantages and the inherent difficulties of gene introduction, it is speculated that the components after exogenous DNA digestion play a greater role in mutagenesis.