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采用直接逆转录一多聚酶链反应（ＲＴ－ＰＣＲ）对加标海水、海泥、毛蚶的浓缩样品，用不同消毒方法处理过的甲型肝炎病毒（ＨＡＶ）及毛蚶实测样品进行了ＨＡＶ－ＲＮＡ分析。结果表明，该方法可检出加标海水、海泥及毛蚶中的ＨＡＶ－ＲＮＡ；对苯酚消毒灭活的ＨＡＶ检验结果表明含有完整核酸序列的灭活病毒也能用ＲＴ－ＰＣＲ方法检出；毛蚶实测样品结果表明，只有１９８８年上海毛蚶ＲＴ－ＰＣＲ阳性，但细胞培养间接免疫荧光法检测结果为阴性，说明毛蚶冻存太久（≥５年），ＨＡＶ已不具感染性。直接ＲＴ－ＰＣＲ方法简便敏感，不需超速离心及其它特殊设备，在普通实验室也能使用。
HAV-RNA analysis was performed on samples of concentrated seawater, sea mud and hairy mildew using direct reverse transcription-polymerase chain reaction (RT-PCR), samples of hepatitis A virus (HAV) treated with different disinfection methods, . The results showed that this method could detect HAV-RNA in spiked seawater, sea mud and pomfret. HAV test showed that the inactivated virus containing the complete nucleic acid sequence could also be detected by RT-PCR. The results of real test samples showed that only RT-PCR was positive in 1988, but indirect immunofluorescence was negative in cell culture, indicating that it had been frozen for too long (≥5 years) and HAV was not infectious. The direct RT-PCR method is simple and sensitive, does not require ultracentrifugation and other special equipment, can also be used in ordinary laboratories.