为研究人巨噬细胞的离子通道及其调控元件是否参与了抗结核分枝杆菌感染免疫 ,利用表达谱芯片技术研究细菌感染后宿主巨噬细胞基因的表达情况。在全局表达谱分析的基础上 ,重点分析了离子通道及其调控元件的表达 ,并比较无毒株和临床分离有毒株在诱导离子通道及其调控元件表达方面的差异。结果表明 ,细菌感染影响离子通道及其调控元件基因的表达 ,涉及的离子通道包括钾通道、钠通道、氯通道、钙通道 ,差异表达的调控元件包括G蛋白、G蛋白偶联受体、蛋白质激酶和磷酸化酶 ;临床株感染影响的离子通道及其调控元件较无毒株广泛和丰富。这些观察结果提示 ,离子通道及其调控元件参与了宿主细胞对感染细菌的免疫应答 ,有关离子通道及其调控元件在抗结核免疫中的作用有待进一步研究。芯片研究的结果为将来的研究提供了线索 To investigate whether the ion channels of human macrophages and their regulatory elements are involved in the immunization against Mycobacterium tuberculosis, the expression of host macrophage genes after bacterial infection was studied by using the expression profiling microarray. Based on the analysis of global expression profiles, the expression of ion channels and their regulatory elements was analyzed. The differences in the expression of ion channels and their regulatory elements between non-toxic and clinically isolated strains were compared. The results showed that the bacterial infection affected the expression of ion channels and their regulatory elements. The involved ion channels include potassium channel, sodium channel, chloride channel and calcium channel. The differentially expressed regulatory elements include G protein, G protein-coupled receptor, protein Kinases and phosphorylases. The ion channels and their regulatory elements influenced by clinical infection are more extensive and abundant than non-toxic ones. These observations suggest that ion channels and their regulatory elements are involved in the immune response of host cells to infected bacteria. The role of ion channels and their regulatory elements in anti-TB immunity remains to be further studied. The results of the chip research provide clues for future research