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目的观察吴茱萸次碱对大鼠脑缺血-再灌注损伤及脑组织降钙素基因相关肽的影响。方法大鼠实验前30min静脉注射吴茱萸次碱(50、100、300μg·kg-1),然后用线栓法制作大鼠局灶性脑缺血-再灌注损伤模型,缺血2h,再灌注24h。于再灌注后6、12、24h进行神经功能缺陷评分;采用TTC染色法测定脑梗死体积;放射免疫法检测脑组织匀浆中降钙素基因相关肽(CGRP)含量。结果吴茱萸次碱呈剂量依赖性减少脑梗死体积并改善功能预后,与溶媒组比较有显著性差异;各吴茱萸次碱治疗组脑梗死后CGRP含量和溶媒组比较显著增高。结论吴茱萸次碱对缺血性脑损伤有明显保护作用,其机制可能与促进脑组织CGRP的释放有关。
Objective To observe the effect of rutacarpine on cerebral ischemia-reperfusion injury and calcitonin gene-related peptide in brain tissue of rats. METHODS: Rats were treated with intravenous injection of rutacarpine (30, 100, 300 μg·kg-1) 30 min before the experiment, and then they were treated with suture method to establish a model of focal cerebral ischemia-reperfusion injury in rats. The rats were sacrificed for 2 h and 24 h after reperfusion. . Neurological deficit scores were performed at 6, 12, and 24 hours after reperfusion. The volume of cerebral infarction was measured by TTC staining. The content of calcitonin gene-related peptide (CGRP) in brain homogenates was measured by radioimmunoassay. RESULTS: Rutaecarpine reduced the infarct volume and improved the functional prognosis in a dose-dependent manner, which was significantly different from that in the vehicle group. The CGRP content in the cerebral infarction group and the vehicle group were significantly higher in each of the rutacarpine-treated groups. Conclusion Rutaecarpine has obvious protective effect on ischemic brain injury. The mechanism may be related to the promotion of CGRP release in brain tissue.