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目的了解南宁市静脉注射吸毒人群(IDUs)中HIV/HCV共感染者HCV基因亚型流行情况。方法收集2012—2014年间南宁市静脉注射吸毒人员HIV/HCV共感染者血浆样本总共108份,设计特异性引物,通过巢式PCR对HCV NS5B区域长339 bp的片段进行扩增并测序,构建系统进化树对HCV进行基因分型。结果 108份血浆样本中巢式PCR成功扩增基因片段102份,系统进化分析显示检出4种HCV基因型含6种基因亚型,各基因亚型所占样本比例为6a 34.31%(35/102)、3b 33.33%(34/102)、1a 20.59%(21/102)、1b 6.87%(7/102)、3a 3.92%(4/102)和2a 0.98%(1/102)。在不同人口学特征时102份样本HCV基因型组成差异无统计学意义(P>0.05)。结论南宁市IDUs人群HIV/HCV共感染者中至少存在6种HCV基因亚型流行,6a、3b和1a为主要流行亚型。
Objective To understand the prevalence of HCV genotype in HIV / HCV co-infected patients (IDUs) in Nanning City. Methods A total of 108 plasma samples collected from HIV / HCV co-infected drug users in Nanning City during 2012-2014 were collected. Specific primers were designed and the 339 bp fragment of HCV NS5B region was amplified and sequenced by nested PCR. Evolutionary trees genotype HCV. Results A total of 102 gene fragments were amplified by nested PCR in 108 plasma samples. Phylogenetic analysis showed that the genotypes of the four HCV genotypes were 6 genotypes and the genotypes were 634.31% (35 / 102, 33.33% (34/102), 1a 20.59% (21/102), 1b 6.87% (7/102), 3a 3.92% (4/102) and 2a 0.98% (1/102). There was no significant difference in the genotypes of HCV among the 102 samples at different demographic characteristics (P> 0.05). Conclusions There are at least 6 HCV genotypes among HIV / HCV co-infected patients in IDUs of Nanning. 6a, 3b and 1a are the major epidemic subtypes.