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为研究维生素E对2,3,7,8-四氯二苯并二噁英(TCDD)染毒小鼠生殖内分泌功能和卵巢的影响,设计了急性染毒实验。将24只4-5周龄的健康雌性小鼠(Mus musculus)随机分为对照组、TCDD(30μg/kg)染毒组和染毒同时给予100mg/kg维生素E组3组。通过放射免疫法测定小鼠血浆孕酮、雌二醇含量的变化,显微镜下计数卵泡数量并观察其形态变化。结果表明,TCDD能导致小鼠血浆孕酮含量的降低及卵巢结构的紊乱、卵泡数量的减少。100mg/kg的维生素E能明显对抗急性TCDD染毒引起的雌鼠孕酮含量和卵泡数量降低,缓解TCDD引起的小鼠卵巢形态结构的病变;TCDD染毒和维生素E对小鼠血浆雌二醇含量均没有明显影响。结果表明,维生素E对TCDD引起的内分泌功能紊乱以及卵泡数量降低有拮抗作用。
In order to study the effects of vitamin E on reproductive and endocrine function and ovarian function in 2,3,7,8-tetrachlorodibenzodioxin (TCDD) -induced mice, an acute exposure experiment was designed. Twenty four healthy female mice (4-5 weeks old) (Mus musculus) were randomly divided into control group, TCDD (30μg / kg) group and 100mg / kg vitamin E group. The contents of progesterone and estradiol in plasma were measured by radioimmunoassay. The number of follicles was counted under microscope and the morphological changes were observed. The results showed that TCDD can lead to the decrease of plasma progesterone level in mice and the disturbance of ovarian structure and the reduction of the number of follicles. 100mg / kg of vitamin E can significantly prevent the acute pregnancy induced by TCDD female progesterone content and the number of follicles reduced TCDD-induced ovarian morphological changes in mice lesions; TCDD exposure and vitamin E in mice plasma estradiol No significant effect on the content. The results show that vitamin E on TCDD-induced endocrine disorders and reduce the number of follicles antagonistic effect.