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目的:采用HPLC-DAD-ELSD法测定伤痛酊(STD)中氯化两面针碱、乙氧基白屈菜红碱、岩白菜素和百两金皂苷A的含量。方法:采用Hypersil C18色谱柱(200 mm×4.6 mm,5μm);流速:0.8 ml·min-1;氯化两面针碱和乙氧基白屈菜红碱:流动相A为乙腈,流动相B为0.1%甲酸-三乙胺(pH 4.5),检测波长为273 nm。岩白菜素和百两金皂苷A:甲醇-水(25∶75)为流动相;漂移管温度:95℃,载气(N2)流速:2.5 SLPM·min-1。结果:氯化两面针碱与乙氧基白屈菜红碱进样量分别在0.021~0.426μg(r=0.999 5)、0.075~1.494μg(r=0.999 8)与峰面积呈良好的线性关系,平均加样回收率分别为99.22%、98.61%,RSD分别为0.64%、0.46%(n=9);岩白菜素和百两金皂苷A分别在0.215~4.304(r=0.999 3)、0.286~5.728μg(r=0.999 7)进样量的自然对数值与峰面积的自然对数值良好的线性关系,平均加样回收率分别为99.15%、99.25%,RSD分别为0.77%、0.56%(n=9)。结论:该测定方法结果准确、灵敏度高、重复性好,可用于伤痛酊中氯化两面针碱、乙氧基白屈菜红碱、岩白菜素和百两金皂苷A的含量测定。
OBJECTIVE: To determine the content of nitidine chloride, ethionine chelerythrine, bergenin, and biochemical saponin A in Tongye tincture (STD) by HPLC-DAD-ELSD. METHODS: Hypersil C18 column (200 mm × 4.6 mm, 5 μm) was used. The flow rate was 0.8 ml · min -1. The nitidine chloride and ethoxysilverine were extracted by mobile phase A, mobile phase A 0.1% formic acid - triethylamine (pH 4.5), the detection wavelength was 273 nm. Bergenin and 100% gold saponin A: methanol-water (25:75) as the mobile phase; drift tube temperature: 95 ° C; carrier gas (N2) flow rate: 2.5 SLPM · min-1. Results: The injection volume of nitidine chloride and ethionine was higher than 0.021-0.426μg (r = 0.999 5) and 0.075-1.494μg (r = 0.999 8) respectively, and the peak area showed a good linear relationship , And the average recoveries were 99.22% and 98.61%, respectively. The RSDs were 0.64% and 0.46%, respectively (n = 9). The concentrations of bergenin and erythrosin A were 0.215 ~ 4.304 ~ 5.728μg (r = 0.999 7). The average logarithm of the injected sample was linear with the logarithm of the peak area. The average recoveries were 99.15% and 99.25%, respectively. The RSDs were 0.77% and 0.56% n = 9). Conclusion: The method is accurate, sensitive and reproducible. It can be used for the determination of nitidine chloride, ethionine chelidonine, bergenin and bioconazole saponin A in Tongtong tincture.