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[目的]探讨DNA修复能力与1,3-丁二烯(BD)职业暴露致外周血淋巴细胞遗传学损伤的关联性。[方法]收集个人职业史、年龄、性别、吸烟和饮酒状况等信息,气相色谱法检测作业环境的BD浓度,利用染色体断裂试验评价60名职业BD暴露工人和60名非暴露工人的外周血淋巴细胞对诱变剂博莱霉素所致DNA损伤的修复能力。[结果]作业区空气中BD浓度为1.8(0.59~2.76)mg/m3。职业BD暴露组染色体断裂率[(1.06±0.41)%]高于对照组[(0.85±0.36)%,P<0.01]。职业BD暴露人群中饮酒者的b/c值高于不饮酒者(P<0.05)。[结论]DNA修复能力的下降可能是BD致癌过程中的重要生物学事件。
[Objective] To investigate the relationship between DNA repair ability and genetic damage of peripheral blood lymphocytes caused by 1,3-butadiene (BD) occupational exposure. [Methods] Personal information such as occupational history, age, sex, smoking and drinking status were collected. Gas chromatography was used to detect the BD concentration in the working environment. Chromosome breakage test was used to evaluate the peripheral blood lymph of 60 professional BD workers and 60 non-exposed workers The ability of cells to repair DNA damage caused by the mutagen bleomycin. [Result] The BD concentration in the air of work area was 1.8 (0.59 ~ 2.76) mg / m3. The chromosome breakage rate in the occupational BD exposure group was (1.06 ± 0.41)% higher than that in the control group (0.85 ± 0.36%, P <0.01). The drinkers in the occupational BD exposure group had higher b / c values than non-drinkers (P <0.05). [Conclusion] The decrease of DNA repair ability may be an important biological event in carcinogenesis of BD.