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目的:以聚类分析、主成分分析等方法,结合含量测定,建立并完善藤菔降压片评价体系。方法:流动相为乙腈-0.01 mol/L-1磷酸二氢钠水溶液梯度洗脱,检测波长224 nm。对10批次不同时间制备的藤菔降压片进行测定,建立了藤菔降压片标准指纹谱,并计算各批次的相似度;对所得指纹图谱数据进行聚类分析及主成分分析。结果:方法精密度、重复性与稳定性良好。将藤菔降压片聚类为1类,确定21个共有峰,样品相似度>0.95,主成分分析显示10批藤菔降压片无明显分类。结论:建立的HPLC指纹图谱、聚类分析和主成分分析方法有助于完善藤菔降压片质量评价体系。
OBJECTIVE: To establish and improve the evaluation system of rattan buck antihypertensive tablets by cluster analysis, principal component analysis and other methods. Methods: The mobile phase consisted of a gradient elution of acetonitrile-0.01 mol / L sodium dihydrogen phosphate aqueous solution at a detection wavelength of 224 nm. Ten batches of decoction of decoction of Fujimi were prepared at different times. The standard fingerprint of decoction of decoction of Fujiminori was established, and the similarity of batches was calculated. The fingerprint and fingerprint data were analyzed by cluster analysis and principal component analysis. Results: The method has good precision, repeatability and stability. The Fujikaki buckets were clustered into 1 category, 21 common peaks were identified, and the similarity of the samples was> 0.95. The principal component analysis showed that there was no obvious classification for the 10 batches of Fujimi antihypertensive tablets. Conclusion: The HPLC fingerprinting, cluster analysis and principal component analysis are helpful to improve the quality evaluation system of rattan buckets.