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目的:探讨高住高练低训(HiHiLo)对红细胞调节T淋巴细胞免疫能力的影响机制。方法:以16名北京体育大学足球专项运动员为对象,实验组(HiHiLo组)和对照组(低住低训组,LoLo组)各8名。实验组每晚入住低氧房(O2浓度14.2%,相当于海拔3000米高度)10小时,每周在低氧房内进行2次72%最大摄氧量蹬功率自行车30分钟训练;对照组平原居住,每周进行2次平原80%最大摄氧量蹬功率自行车30分钟训练。两组每周进行3次由同一教练执教的专项训练。实验期4周。分别于实验前、入住10小时后、实验2、3、4周末清晨取静脉血,与相应的荧光标记抗体反应,采用流式细胞仪记录红细胞CD58、CD59平均荧光强度及T淋巴细胞CD2阳性细胞率。结果:4周实验后,实验组和对照组CD58表达分别较实验前下降了33.14%和30.29%(均P<0.01),两组间有显著性差异(P<0.05);CD59表达分别下降了10.38%和9.68%(均P<0.01),两组间无显著性差异(P>0.05);T淋巴细胞CD2阳性率在实验期无明显变化,两组间也未见显著性差异(P>0.05)。结果表明,HiHiLo和LoLo均可影响人体红细胞CD58、CD59表达,但HiHiLo的影响作用有更明显的趋势,而T淋巴细胞CD2表达受低氧和/或训练刺激的影响不明显。
Objective: To investigate the mechanism of HiHiLo on immune function of erythrocyte-mediated T lymphocytes. Methods: Sixteen athletes from Beijing Sport University were enrolled in this study. Eight HiHiLo and eight LoLo patients were selected as experimental subjects. Experimental group night stay in hypoxia room (O2 concentration 14.2%, equivalent to 3000 meters above sea level) for 10 hours a week in the hypoxia room for 72% of the maximum oxygen uptake power bike cycling training for 30 minutes; control group plain Living, 2 times a week 80% of the maximum oxygen uptake pedal power bike 30 minutes training. The two groups were trained three times a week by the same coach. Experimental period of 4 weeks. Respectively before the experiment, 10 hours after admission, venous blood samples were taken at the beginning of the experiment 2,3,4 weeks and reacted with the corresponding fluorescent labeled antibody. The mean fluorescence intensity of CD58 and CD59 on erythrocytes and the CD2 positive cells of T lymphocytes were recorded by flow cytometry rate. Results: After 4 weeks, the expression of CD58 in the experimental group and the control group decreased by 33.14% and 30.29%, respectively (all P <0.01), and there was a significant difference between the two groups (P <0.05) 10.38% and 9.68%, respectively (all P <0.01). There was no significant difference between the two groups (P> 0.05). The positive rate of CD2 in T lymphocytes did not change significantly during the experimental period, 0.05). The results showed that both HiHiLo and LoLo could affect the expression of CD58 and CD59 in human erythrocytes, but the effect of HiHiLo had a more obvious trend. However, the expression of CD2 in T lymphocytes was not significantly affected by hypoxia and / or training stimulation.