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病毒某调节蛋白或酶的表达及研究，无论是从基础理论研究方面还是制备针对该蛋白的特异性杂交瘤进而制备单链可变区抗体进行细胞内免疫基因治疗均有重要的意义．本文用分子克隆法进行HBV多聚酶基因组中终端蛋白（terminaldomain）及逆转录酶（reverstranscrlptasedo－main）基
Virus regulation of a regulatory protein or enzyme expression and research, both from the basic theoretical research or preparation of specific hybridomas for the protein and then prepare single-chain variable region antibodies for intracellular immune gene therapy are of great significance. In this paper, molecular cloning of HBV polymerase in the terminal protein domain (terminaldomain and reverse transcriptase (reverstranscrlptasedo-main