目的探讨青少年抑郁情绪与5-羟色胺转运体基因启动子区多态性(5-HTTLPR)及生活事件之间的关系,为青少年精神卫生领域研究提供实证依据。方法于2013年9月至2014年1月期间,随机选取兰州市537名健康中学生开展抑郁量表(CES-D)、青少年生活事件自评量表(ASLEC)等问卷调查,并刮取口腔黏膜进行DNA提取及5-HTTLPR基因分型。结果中学生5-HTTLPR的SS,SL和LL 3种基因型检出率分别为56.43%,36.31%,7.26%。女生抑郁得分(42.12±10.29)高于男生(38.40±8.83)(t=-4.438,P<0.01)。5-HTTLPR 3种基因型抑郁得分差异无统计学意义(F=1.998,P=0.137)。生活事件对男、女生抑郁情绪有影响(t值分别为-5.159,-7.720,P值均<0.01),未发现5-HTTLPR基因型和生活事件对男、女生抑郁情绪存在交互作用(F值分别为1.147,1.133,P值均>0.05)。结论 5-HTTLPR与健康青少年抑郁情绪相关性还需进一步探讨。 Objective To investigate the relationship between depression and serotonin transporter gene promoter region polymorphism (5-HTTLPR) and life events in adolescents, and to provide empirical evidence for the research of adolescent mental health. Methods From September 2013 to January 2014, 537 healthy middle school students in Lanzhou were randomly selected from questionnaires of depression scale (CES-D) and adolescent life event self-rating scale (ASLEC), and scalded oral mucosa DNA extraction and 5-HTTLPR genotyping. Results The detection rates of SS, SL and LL genotypes of 5-HTTLPR in middle school students were 56.43%, 36.31% and 7.26% respectively. The depression score of female students (42.12 ± 10.29) was higher than that of boys (38.40 ± 8.83) (t = -4.438, P <0.01). There was no significant difference in depression scores between 5-HTTLPR and 3 genotypes (F = 1.998, P = 0.137). The life events affected the depression of boys and girls (t = -5.159, -7.720, P <0.01 respectively). There was no interaction between depression and 5-HTTLPR genotype and life events (F value Respectively, 1.147,1.133, P values ​​were> 0.05). Conclusion The relationship between 5-HTTLPR and depression in healthy adolescents needs to be further explored.