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目的探讨缺血预处理对心肌缺血耐受的影响。方法将28只SD大鼠随机分为缺血预处理组和对照组,每组14只。两组大鼠复制离体心脏灌注动物模型,缺血预处理组采用5min缺血、10min灌注,反复3次;对照组大鼠常温灌注45min,2组大鼠均心脏常温缺血30min。测定2组大鼠心脏缺血后跳动时间,于缺血前和缺血30min测定心肌ATP含量、Na+-K+-ATP酶活性。结果缺血预处理组心脏缺血后跳动时间较对照组明显延长[(14.3±3.6)minvs.(9.5±2.5)min,P<0.05],缺血后心肌ATP含量低于对照组[(275±43)nmol/gvs.(358±65)nmol/g,P<0.05],但缺血后2组间Na+-K+-ATP酶活性的差异无统计学意义。结论缺血预处理能提高心肌能量利用能力,延长缺血后心脏缺血跳动时间。
Objective To investigate the effect of ischemic preconditioning on myocardial ischemic tolerance. Methods Twenty-eight SD rats were randomly divided into ischemic preconditioning group and control group, with 14 rats in each group. Rats in the two groups were implanted with isolated heart perfusion. The rats in the ischemic preconditioning group were subjected to ischemia for 5 minutes and perfusion for 10 minutes. The rats in the control group were infused for 45 minutes at room temperature. Rats in both groups were subjected to ischemia for 30 minutes at room temperature. The beating time of the heart was measured in the two groups. ATP content and Na + -K + -ATPase activity were measured before ischemia and 30min after ischemia. Results The duration of ischemic preconditioning in ischemic preconditioning group was significantly longer than that in control group [(14.3 ± 3.6) min vs (9.5 ± 2.5) min, P <0.05] ± 43) nmol / gvs (358 ± 65) nmol / g, P <0.05]. However, there was no significant difference in Na + -K + -ATPase activity between the two groups after ischemia. Conclusion Ischemic preconditioning can improve myocardial energy utilization and prolong ischemic heartbeat time after ischemia.