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为探讨长期不同水平维生素Ａ对大鼠细胞免疫功能的影响，采用在８周内给予大鼠进食不同水平维生素Ａ的饲料，并测定其外周血Ｔ细胞亚群、淋巴细胞转化及ＩＬ－２和肿瘤坏死因子－α（ＴＮＦ－α）的分泌。结果显示：①维生素Ａ轻、重度缺乏均会导致ＣＤ４细胞减少，ＣＤ４／ＣＤ８下降，ＩＬ－２及ＴＮＦ－α分泌减少。重度维生素Ａ缺乏还导致胸腺、脾脏明显萎缩，淋巴细胞转化受抑。②维生素Ａ５倍过量组ＣＤ４细胞增多，ＣＤ４／ＣＤ８上升，淋巴细胞转化活跃，ＩＬ－２分泌增加。③维生素Ａ２５倍过量组则显示ＣＤ８细胞增多，ＣＤ４／ＣＤ８下降。结果提示，维生素Ａ缺乏或长期较大剂量的使用均可造成免疫功能的损害，而在一定时间内（８周）使用适当剂量的维生素Ａ（５倍建议膳食量）可能对免疫功能有促进作用
In order to investigate the effect of long-term different levels of vitamin A on cellular immunity in rats, the rats were fed with different levels of vitamin A within 8 weeks. The levels of T lymphocyte subsets, lymphocyte transformation and IL-2 and Tumor necrosis factor-α (TNF-α) secretion. The results showed that: (1) Both light and severe vitamin A deficiency resulted in decreased CD4, decreased CD4 / CD8 and decreased IL-2 and TNF-α secretion. Severe vitamin A deficiency also led to a significant atrophy of the thymus, spleen, suppressed lymphocyte transformation. (2) In CD4 + CD3 + cells, CD4 + CD8 + cells increased, lymphocytes became more active and IL-2 increased. ③ vitamin A25-fold over the group showed an increase in CD8 cells, CD4 / CD8 decreased. The results suggest that vitamin A deficiency or long-term use of larger doses can cause damage to immune function, and the use of the appropriate dose of vitamin A (5-fold the recommended dietary amount) within a certain period of time (8 weeks) may have an effect on immune function