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目的研究缺氧对HSC基质金属蛋白酶Ⅱ（matrixmetalloproteinase-2，MMP-2）表达及活性调节的影响。方法大鼠HSC体外原代培养，以免疫细胞化学及ELISA法分别检测细胞内MMP－2及基质金属蛋白酶组织抑制因子Ⅱ（Tissueinhibitorofmetalloproteinade－2、TIMP－2）的表达，和培养上清液中两者的相对量,并以酶图活测培养上清液MMP－2活力。结果缺氧培养12h，免疫细胞化学显示MMP－2表达高于对照组（t＇＝3．4685，P＜0．05），TIMP－2表达则降低（t＇＝2．5673，P＜0．05）；培养上清液中MMP－2酶活性低于对照组（t＇＝4.270,P＜0.01）。缺氧6h上清液MMP－2蛋白相对含量增加值高于12h及24h组（t值分别为4.0945、2.4652;P值分别为＜0.001、0.025）；TIMP－2变化趋势与MMP－2则相反，但各组间差异无显著性。结论缺氧使肝星状细胞MMP－2含量增加，该影响在早期更明显
Objective To investigate the effect of hypoxia on the expression and activity of matrix metalloproteinase-2 (MMP-2) in HSC. Methods HSCs were primarily cultured in vitro. The expressions of MMP-2 and TIMP-2 were detected by immunocytochemistry and ELISA respectively. The expression of TIMP-2 and TIMP- The relative amount of the person, and enzymatic activity of the culture supernatant MMP-2 activity. Results After hypoxia for 12 hours, the expression of MMP-2 was higher in immunocytochemistry than in the control group (t ’= 3.4685, P <0.05) and TIMP-2 expression was lower .05). The activity of MMP-2 in culture supernatant was lower than that in control group (t ’= 4.270, P <0.01). The relative content of MMP-2 in supernatant of hypoxia 6h was higher than that of 12h and 24h (t = 4.0945,2.4652, P <0.001,0.025, respectively). The change of TIMP-2 was opposite to that of MMP-2 , But no significant difference between the groups. Conclusion Hypoxia increases the content of MMP-2 in hepatic stellate cells, which is more obvious in early stage