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目的评估串珠素小干扰RNA(siRNA)对肿瘤细胞增殖的影响。方法体外培养HCT15和HT29结直肠癌细胞,将构建好的串珠素siRNA载体转染培养细胞中,荧光显微镜下观察转染效率,qRT-PCR法检测串珠素mRNA表达,Western blot法检测串珠素蛋白表达,MTS法检测串珠素siRNA对肿瘤细胞增殖的影响。结果串珠素siRNA转染肿瘤细胞48 h后,肿瘤细胞串珠素mRNA、蛋白水平均出现降低(P<0.05)。串珠素siRNA对肿瘤细胞增殖有明显的抑制作用(P<0.01)。结论串珠素siRNA对结直肠癌肿瘤细胞增殖具有显著的抑制效果。
Objective To evaluate the effect of beaded small interfering RNA (siRNA) on tumor cell proliferation. Methods HCT15 and HT29 colorectal cancer cells were cultured in vitro. The constructed siRNA vector was transfected into cultured cells. The transfection efficiency was observed under a fluorescence microscope. The expression of perlecan mRNA was detected by qRT-PCR. MTS method was used to detect the effect of bezin siRNA on the proliferation of tumor cells. Results The mRNA and protein levels of perlecan all decreased after beaded with siRNA for 48 h (P <0.05). Perlecan siRNA significantly inhibited tumor cell proliferation (P <0.01). Conclusion Bezin siRNA has a significant inhibitory effect on the proliferation of colorectal cancer cells.