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采用反相高效液相色谱法检测黄鳝血液中阿苯哒唑及其3种代谢产物(阿苯哒唑亚砜、阿苯哒唑砜、阿苯哒唑氨基砜)的残留。结果表明:选用C18色谱柱,紫外检测器,波长为292 nm,样品经乙酸乙酯2次提取,以甲醇-冰乙酸-水为流动相,在浓度为0.1~20μg/mL的范围内,标准曲线线性良好,检测限分别为0.02、0.03、0.05和0.05μg/mL,回收率78.20%~92.35%,重现性较好。
The residues of albendazole and its three metabolites (albendazole sulfoxide, albendazole sulfone and albendazole sulfamido sulfone) in the blood of the eel were detected by RP-HPLC. The results showed that C18 column and UV detector were used at a wavelength of 292 nm. The sample was extracted with ethyl acetate twice and methanol - glacial acetic acid - water was used as the mobile phase at a concentration of 0.1 ~ 20 μg / mL. The standard The curves showed good linearity. The detection limits were 0.02, 0.03, 0.05 and 0.05 μg / mL, respectively. The recoveries were 78.20% ~ 92.35%. The reproducibility was good.