本文报告以高铁血红蛋白(Met-Hb)微量组织化学洗脱法筛选了上海地区1,000例新生儿脐血标本。阳性者并以分光光度法定量测定葡萄糖-6-磷酸脱氢酶(简称G-6-PD)活性为对照。1,000例新生儿红细胞G-6-PD缺陷发病率为0.2％(男、女各1例).该2例临床均无黄疸。家系调查发现,男性患儿之兄亦为G-6-PD缺陷者,目前有核黄疸后遗症。我室亦曾检查87例新生儿高胆红素血症,其中2例为G-6-PD缺陷引起.因此,我们认为上海地区新生儿高胆红素血症病人中,G-6-PD缺陷并非重要原因. This paper reports the methemoglobin (Met-Hb) trace chemiluminescence screening of 1,000 neonatal cord blood samples in Shanghai. Positive and spectrophotometric determination of glucose-6-phosphate dehydrogenase (referred to as G-6-PD) activity as a control. 1,000 cases of neonatal erythrocyte G-6-PD defect incidence of 0.2% (male and female in 1 case) .The two cases were clinically without jaundice. Pedigree survey found that male children’s brother is also G-6-PD defects, there are currently cases of nuclear jaundice sequelae. We also examined 87 cases of neonatal hyperbilirubinemia, of which 2 cases were caused by G-6-PD deficiency.Therefore, we believe that in Shanghai neonates with hyperbilirubinemia, G-6-PD Defects are not an important reason.