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目的:考察冬虫夏草对体内PM2.5超细颗粒物排出的影响和机制探讨。方法:对斑马鱼卵黄囊内注射纳米活性炭颗粒制备斑马鱼PM2.5超细颗粒物排出模型,分别给予培植冬虫夏草和野生冬虫夏草干预后,观察其对纳米颗粒从斑马鱼体内排出进入肠道的影响;采用静脉注射给予纳米活性炭建立斑马鱼PM2.5超细颗粒物吞噬模型,分别给予培植冬虫夏草和野生冬虫夏草干预后,观察其对斑马鱼巨噬细胞吞噬PM2.5能力的影响;使用静脉注射墨汁的方法,测试培植冬虫夏草和野生冬虫夏草对小鼠巨噬细胞吞噬功能的影响。结果:与模型组比较,培植冬虫夏草20、60μg/mL和野生冬虫夏草60μg/mL均具有显著的促进纳米颗粒排出进入肠道的作用(P<0.05或P<0.01);与模型组比较,培植冬虫夏草6、20、60μg/mL组和野生冬虫夏草60μg/mL吞噬了纳米活性炭的巨噬细胞数量均显著增加(P<0.05或P<0.01);与正常对照组比较,培植冬虫夏草200、600、1800 mg/kg组和野生冬虫夏草600、1800 mg/kg组均能显著增加小鼠的吞噬指数(P<0.05或P<0.01)。结论:培植冬虫夏草和野生冬虫夏草均具有促进PM2.5超细颗粒物排出的作用,其机制可能与增强巨噬细胞吞噬功能有关。
Objective: To investigate the influence of Cordyceps on the excretion of PM2.5 ultrafine particles in vivo and to explore its mechanism. Methods: The zebrafish PM2.5 superfine particles were injected into the yolk sac of zebra fish to produce ultrafine PM2.5 particles. The effects of different concentrations of nanoparticles on the intestinal tract were observed after intervention with Cordyceps sinensis and wild Cordyceps sinensis respectively. The model of phagocytosis of zebrafish PM2.5 superfine particles was established by intravenous injection of nano-activated carbon. The effects of culturing the cultured cordyceps sinensis and wild Cordyceps sinensis on the PM2.5 phagocytosis of zebrafish macrophages were observed. Using the method of intravenous injection of ink , Test cultivated Cordyceps sinensis and wild Cordyceps on mouse macrophage phagocytosis. Results: Compared with the model group, cultivation of Cordyceps Sinensis 20,60μg / mL and wild Cordyceps Sinensis 60μg / mL significantly promoted the release of nanoparticles into the intestine (P <0.05 or P <0.01); compared with the model group, Cordyceps sinensis The numbers of macrophages phagocytosed with nanometer activated carbon in 6, 20, 60μg / mL group and wild Cordyceps sinensis significantly increased (P <0.05 or P <0.01). Compared with the normal control group, Cordyceps sinensis cultivated 200,600,1800 mg / kg group and wild Cordyceps 600,1800 mg / kg group can significantly increase the phagocytic index of mice (P <0.05 or P <0.01). CONCLUSION: Cordyceps sinensis and wild Cordyceps sinensis can both promote the excretion of PM2.5 ultrafine particles. The mechanism may be related to the enhancement of macrophage phagocytosis.