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目的：测定人血清中特布他林浓度，研究中国男性健康志愿者口服该药后药代动力学规律。方法：用ＬＣＭＳ法测定特布他林浓度。流动相：２％冰乙酸—异丙醇（９８５∶１５）；色谱柱：ＳｈｉｍａｄｚｕＶＰＯＤＳ；流速：０５ｍＬ·ｍｉｎ－１；柱温：３０℃。结果：特布他林浓度在０２～２０ｎｇ·ｍＬ－１范围内线性关系良好（γ＝０９９９８）。方法的回收率高于９０％，最低浓度的日内及日间精密度分别为８２％和１２９％，最低检测限为００５ｎｇ。１０名健康志愿者单剂量口服特布他林口服液７５ｍｇ后，药代动力学行为符合二房室模型。结论：此法灵敏度高、选择性强，符合生物样品分析要求。
OBJECTIVE: To determine the concentration of terbutaline in human serum and study the pharmacokinetics of the drug in Chinese male healthy volunteers. Methods: The determination of terbutaline by LC MS method. Mobile phase: 2% glacial acetic acid-isopropanol (985:15); column: ShimadzuVP-ODS; flow rate: 0.5mL · min-1; column temperature: 30 ℃. Results: Terbutaline concentration ranged from 02 to 20 ng · mL-1. The linear relationship was good (γ = 09998). The recovery rate of the method was higher than 90%. The intra- and inter-day precision of the lowest concentration were 8.2% and 12.9%, respectively. The lowest detection limit was 0. 05ng. Ten healthy volunteers oral single dose of terbutaline oral 5mg, pharmacokinetic behavior in line with two-compartment model. Conclusion: This method has high sensitivity and strong selectivity, which meets the requirements of biological sample analysis.