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选取6周龄、雌雄各半、体重18~22 g的SPF级昆明小鼠(Mus musculus)100只,随机分成5组,每组4个重复,每个重复5只小鼠。分别用含量为40%和60%的转Bar基因稻谷Bar68-1和非转基因稻谷D68日粮喂养小鼠,亲代小鼠饲养180 d后开始繁殖子一代(F1),每代小鼠饲养180 d。在180 d后分别从亲代和子一代每组随机抽取5只小鼠,提取肠道内容物基因组DNA,利用细菌通用引物对细菌16S r DNA的V3区序列进行PCR扩增,并将扩增产物用变性梯度凝胶电泳(denaturing gradient gel electrophoresis DGGE)。研究显示,各组小鼠肠道菌群相似性系数无显著差异(P>0.05);肠道主要优势菌群相同。结果表明,转Bar基因稻谷对小鼠肠道菌群的作用不明显。
One hundred SPF Kunming mice (Mus musculus), 6 weeks old, male and female, weighing 18-22 g, were randomly divided into 5 groups with 4 replicates in each group and 5 mice in each replicate. The first generation (F1) of F1 generation was started after 180 d of feeding with the Bar68-1 transgenic Bar and the non-transgenic D68 JEV feeding, respectively. Each generation of mice was maintained for 180 days . After 180 days, five mice were randomly selected from each parental and offspring generation to extract the genomic DNA of the intestine. PCR was used to amplify the V3 region of the bacterial 16S r DNA using bacterial universal primers. Denaturing gradient gel electrophoresis (DGGE). The study showed that there was no significant difference in the similarity coefficient of intestinal flora among mice in each group (P> 0.05). The dominant intestinal flora was the same. The results showed that the effect of Bar transgenic rice on mouse intestinal flora was not obvious.