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目的 脱氧核苷是构成 DNA分子的重要组分 ,通过补充脱氧嘌呤和嘧啶核苷探讨对 DNA损伤修复的影响。方法 采用“彗星”电泳技术分析 DNA断裂损伤 ,用 1 0 0 μmol/LH2 O2 诱导人体外周血淋巴细胞 DNA损伤后分为两组 :一组为对照组 ,另一组为脱氧核苷补充组( 2 0 μl,1 0 -4 mol/L)。结果 对照组淋巴细胞从初始到培养 2 h后 DNA损伤程度逐渐下降 ,由1 67.75下降为 68.1 ( AU) ;而补充脱氧胸苷组 DNA损伤修复能力与对照组相比无明显增高 ,同时补充 DNA合成所必须的四种脱氧核苷经过 3h的培养后也没有出现明显促进 DNA损伤修复的作用。结论 体外补充脱氧核苷对 DNA修复未显示有意义的作用 ,与 DNA损伤修复过程利用脱氧核苷的途径可能不同于 DNA的复制合成有关
Objective Deoxyribonucleosides (DNA) constitute an important component of DNA molecules. The effects of deoxynucleosides and pyrimidine nucleosides on DNA damage repair are discussed. Methods DNA damage was analyzed by “Comet” electrophoresis technique. DNA damage of human peripheral blood lymphocytes was induced by 100 micromol / L H2O2 into two groups: one was control group and the other was DNA-supplemented group 2 0 μl, 1 0 -4 mol / L). Results The DNA damage of lymphocytes in control group decreased gradually from 1 67.75 to 68.1 (AU) after initial 2 h incubation. However, DNA damage repair capacity of the control group was not significantly higher than that of the control group The four kinds of deoxynucleosides necessary for the synthesis did not obviously promote the DNA damage repair after culturing for 3 hours. Conclusion In vitro supplementation with deoxynucleosides did not show any significant effect on DNA repair, and the mechanism of deoxynucleoside utilization during DNA damage repair may be different from that of DNA replication synthesis