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目的初步探讨镍-钴合金磁性纳米晶体作为对比剂在磁共振成像诊断前列腺癌中的作用。方法采用微波水相法合成出镍-钴合金磁性纳米晶体,使用X射线衍射仪、扫描电子显微镜、透射电子显微镜以及量子扰动超导探测仪对所合成的纳米晶体进行表征和测试,分别获得其相应的理化基本性质及磁学性能。将50μg/ml镍-钴合金磁性纳米晶体在培养板底部有/无磁铁吸附的条件下分别与前列腺癌PC-3、DU-145细胞共培养24h后,洗涤并收集培养板中的PC-3、DU-145细胞,并使用3.0T磁共振成像仪分析镍-钴合金磁性纳米晶体在磁共振成像诊断前列腺癌中的横向弛豫增强效应。结果通过微波水相法合成出了尺寸、形貌均一,并可自组装成环状结构的镍-钴合金磁性纳米晶体,该晶体同时具备铁磁性和可用于磁共振成像的超顺磁性,在外加磁场的作用下,材料可“靶向”聚集于前列腺癌细胞,进而有效缩短磁共振成像诊断前列腺癌的横向弛豫时间。结论初步探讨了镍-钴合金磁性纳米晶体作为新型的磁共振成像对比剂,在前列腺癌的“靶向”诊断中可能发挥的重要作用。
Objective To investigate the role of magnetic nanoparticles of nickel-cobalt alloy as a contrast agent in the diagnosis of prostate cancer by magnetic resonance imaging. Methods Nickel-cobalt alloy magnetic nanocrystals were synthesized by microwave aqueous phase method. The synthesized nanocrystals were characterized and characterized by X-ray diffraction, scanning electron microscopy, transmission electron microscopy and quantum perturbation superconducting detector. The corresponding basic physical and chemical properties and magnetic properties. PC-3 and DU-145 cells were co-cultured with PC-3 and DU-145 cells respectively at 50μg / ml nickel-cobalt alloy magnetic nanocrystals with and without magnetite adsorption. The PC-3 , DU-145 cells, and the transverse relaxation enhancement effect of magnetic resonance imaging of nickel-cobalt alloy magnetic nanocrystals in the diagnosis of prostate cancer was analyzed using a 3.0T magnetic resonance imager. Results The nickel-cobalt alloy magnetic nanocrystals with uniform size and morphology and self-assembly into a ring structure were synthesized by microwave aqueous method. The crystals were both ferromagnetic and superparamagnetic for magnetic resonance imaging. Under the action of an external magnetic field, the material can “target ” to accumulate in prostate cancer cells, thereby effectively shortening the transverse relaxation time of magnetic resonance imaging in the diagnosis of prostate cancer. Conclusion The preliminary study of nickel-cobalt alloy magnetic nanocrystals as a novel magnetic resonance imaging contrast agent may play an important role in the diagnosis of prostate cancer.