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目的 :为研究类风湿性关节炎 (RA)患者关节滑膜液浸润的淋巴细胞介导自身免疫病的特性 ,分析了 2 2例RA患者滑膜液中淋巴细胞的免疫表型、对II型胶原的反应频率及IL 10、IL 12的分泌格局。方法 :用流式细胞术分别测定滑膜液和外周血淋巴细胞表型 ,并采用国际标准半有限稀释法分析了关节滑膜液中浸润的淋巴细胞对II型胶原 (CII)的反应频率 ,同时用ELISA方法检测了滑膜液与外周血中IL 10与IL 12含量。结果 :滑膜液中的T淋巴细胞的表型分别为CD4 (39 6 %±10 5 % )和CD8细胞 (36 4 %± 16 4 % ) ,CD4 CD8细胞比值显著低于外周血 ,且同时表达CD16和CD5 6的活化NK细胞占15 5 %± 11 1%。T细胞受体谱取用表明仍以αβTCR为主 (6 9 6 %± 15 7% )。有意义的是 :滑膜液中的T细胞对CII的反应频率为 15 2× 10 - 6 ,远远高于外周血 (4 0× 10 - 6 )。IL 12含量为 (4 19 9± 89 2 )pg ml,IL 10含量为 (187 7± 34 5 )pg ml,与外周血中这 2种细胞因子的含量〔分别为IL 12 :(6 5 32± 34 2 )pg ml和IL 10 :(85± 12 7)pg ml〕比较 ,具有显著的统计学差异。结论 :上述实验结果表明这种具有表达特性的浸润T细胞介导了RA患者关节滑膜组织的免疫损伤。
Objective: To investigate the characteristics of autoimmune diseases mediated by synovial fluid infiltrating synovial fluid in patients with rheumatoid arthritis (RA), the immunophenotypes of lymphocytes in synovial fluid of 22 RA patients were analyzed, Collagen response frequency and IL 10, IL 12 secretion patterns. Methods: The phenotypes of synovial fluid and peripheral blood lymphocytes were determined by flow cytometry. The response frequency of lymphocytes infiltrating with synovial fluid to type II collagen (CII) was analyzed by semi-limited international standard method. The contents of IL 10 and IL 12 in synovial fluid and peripheral blood were detected by ELISA. Results: The T lymphocyte phenotype in synovial fluid was CD4 (39 6% ± 10 5%) and CD8 (36 4% ± 16 4%) respectively. The ratio of CD4 CD8 was significantly lower than that of peripheral blood The activated NK cells expressing CD16 and CD5 6 account for 15 5% ± 11 1%. T cell receptor profiling showed that αβTCR was still predominant (69 6% ± 15 7%). Significantly, the response frequency of T cells to synovial fluid in synovial fluid was 15 2 × 10 - 6, much higher than that in peripheral blood (40 × 10 - 6). IL 12 content was (1919 ± 89 2) pg ml, IL 10 content was (187 7 ± 34 5) pg ml, and the content of these two cytokines in peripheral blood [IL 12: ± 34 2) pg ml and IL 10: (85 ± 12 7) pg ml], with significant statistical differences. Conclusion: The above experimental results show that the expression of infiltrating T cells mediated the synovial tissue of RA patients with immune damage.