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目的:研究精氨酸升压素(4-8)(AVP_(4-8))对来自大鼠大脑皮层和海马的原代培养星状胶质细胞及胚胎神经元细胞中促细胞分裂素活化的蛋白激酶(MAPK)活性变化的影响.同时探讨了这种影响经由的信号传递途径中可能牵涉的蛋白激酶.方法:来自大鼠脑的原代细胞培养于无血清培养基(胚胎神经元细胞)或于给药前无血清饥饿24 h,转入加药物的无钙镁磷酸缓冲液中温育,测定MAPK激酶活性.结果:(1)AVP_(4-8)能刺激原代培养的星状胶质细胞中MAPK活性但对胚胎神经元中MAPK活性没有影响.AVP_(4-8)在星状胶质细胞中诱导的MAPK活性增强作用可被AVP_(4-8)的拮抗剂ZDC(c)PR阻断.(2)MEK(MAPK/ERK激酶)的选择性抑制剂PD98059以及PKC专一性抑制剂GF109203X能消除星状胶质细胞中AVP_(4-8)诱导的活性增强作用.结论:AVP_(4-8)能以受体特异的方式激活星状胶质细胞中的MAPK活性,MEK和PKC这两个激酶参与了这个MAPK信号传递途径.AVP_(4-8)不影响原代培养胚胎神经元中的MAPK活性.
AIM: To investigate the effect of arginine vasopressin (4-8) (AVP_ (4-8)) on the activation of cytokinin in primary cultured astrocytes and embryonic neuronal cells from rat cerebral cortex and hippocampus Protein kinase (MAPK) activity changes.Meanwhile, we investigated the possible involvement of protein kinases in the signal transduction pathway via this pathway.Methods Primary rat brain-derived cells were cultured in serum-free medium (embryonic neuronal cells ) Or serum-starved for 24 h before administration, and then transferred into drug-free calcium-free magnesium phosphate buffer to measure MAPK kinase activity.Results: (1) AVP_ (4-8) stimulated star- MAPK activity in glial cells but had no effect on MAPK activity in embryonic neurons.The enhancement of MAPK activity induced by AVP_ (4-8) in astrocytes was inhibited by ZDC (c ) PR. (2) PD98059, a selective inhibitor of MEK (MAPK / ERK kinase), and GF109203X, a PKC-specific inhibitor, abolished AVP 4-8 -induced activity enhancement in astrocytes. : AVP (4-8) activates MAPK activity in astrocytes in a receptor-specific manner, with two kinases, MEK and PKC, involved in this MAPK No. pathway .AVP_ (4-8) does not affect MAPK activity in primary cultures of embryonic neurons.